Microbiological monitoring in modern intensive livestock systems is of key importance for assessing biological risks, as circulating opportunistic pathogens can form stable communities, act as reservoirs of antibiotic resistance, and pose an epizootic threat. The aim of the study was to analyze the taxonomic composition of microorganisms isolated from farm animals and environmental objects at livestock enterprises in the Omsk Region. Within the conducted microbiological monitoring, 455 samples of biological material from animals and swabs from the surfaces of technological equipment were studied. The material was collected from cattle, pigs, farmed poultry birds. Species identification was performed using classical microbiological methods and modern MALDI-TOF mass spectrometry technology. As a result of the research, 673 pure cultures were isolated and identified. The absolute dominance of the bacterial species Escherichia coli was established (52.45% of the total number of isolates). The second most significant group consisted of representatives of the genus Staphylococcus (19.47%), among which coagulase-negative species (S. epidermidis, S. saprophyticus) predominated, indicating their important role as resident microflora of animal skin and the surfaces of livestock facility infrastructure. The obtained data not only characterize the current microbial profile of typical livestock ecosystems in the region but also form a representative basis for further in-depth study of antibiotic resistance, biofilm-forming potential, and molecular genetic characteristics of the isolated strains. The results of the work are of practical value for improving veterinary and sanitary control systems and monitoring biological risks in modern animal husbandry.
animals, microorganisms, microbiological monitoring, Escherichia coli, Staphylococcus, MALDI-TOF MS
1. Struktura uslovno-patogennoy mikroflory na zhivotnovodcheskih predpriyatiyah razlichnogo profilya // Veterinariya Kubani I M Donnik E N Shilova R A Krivonos 2019 5
2. Variabil'nost' chuvstvitel'nosti uslovnopatogennoy mikroflory k antibakterial'nym sredstvam pri boleznyah telyat // Vestnik APK Verhnevolzh'ya O V Ivanov D Yu Kosterin L E Mel'nikova 2019 4
3. Analiz izmeneniy mikrobioma tolstogo otdela kishechnika svinomatok i porosyat // Agrarnaya nauka A V Filatov D N Ivanov A F Sapozhnikov 2024 11
4. Microbial taxonomy: how and why name changes occur and their significance for (clinical) microbiology // Clinical Chemistry R S Gupta 2022 68
5. Migraciya ptic kak faktor rasprostraneniya infekcionnyh agentov i antibiotikorezistentnyh shtammov mikroorganizmov // Vestnik Omskogo gosudarstvennogo agrarnogo universiteta. 2025. № 2(58) E V Frik N A Lescheva V I Pleshakova
6. Bioplenkoobrazuyuschaya mikroflora v strukture mikroorganizmov, vydelennyh ot sel'skohozyaystvennyh i domashnih zhivotnyh // Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy mediciny im. N.E. Baumana. 2025. T. 261. № 1 I V Antonevskiy V I Pleshakova N A Leschevahttps://doi.org/10.31588/2413-4201-1883-1-261-16
7. Issledovanie bioplenok mikroorganizmov pri disbakteriozah kishechnika u zhivotnyh // Problemy veterinarnoy sanitarii, gigieny i ekologii. 2020. № 1(33) E M Lenchenko D A Blyumenkranchttps://doi.org/10.36871/vet.san.hyg.ecol.202001009
8. Biofilm formation by Salmonella Enteritidis and Salmonella Typhimurium isolated from avian sources is partially related with their in vivo Microbial Pathogenesis K A Borges T Q Furian S N Souza 118 2018 et al.
9. Modifikaciya metoda kolichestvennoy ocenki bioplenkoobrazuyuschey sposobnosti mikroorganizmov s ispol'zovaniem safranina // Uchenye zapiski Kazanskoy akademii veterinarnoy mediciny im I V Antonevskiy V I Pleshakova N A Leschevahttps://doi.org/10.31588/2413-4201-1883-4-264-6 N.E. Baumana. 2025. T. 264. № 4
10. Bakterial'nye bioplenki: rol' v hronicheskih infekcionnyh processah i poisk sredstv bor'by s nimi // Molekulyarnaya genetika, mikrobiologiya i virusologiya T S Romanova Yu M 2021 2
11. Struktura uslovno-patogennoj mikroflory` na zhivotnovodcheskix predpriyatiyax razlichnogo profilya // Veterinariya Kubani I M Donnik E N Shilova R A Krivonos 2019
12. Variabil`nost` chuvstvitel`nosti uslovnopatogennoj mikroflory` k antibakterial`ny`m sredstvam pri boleznyax telyat // Vestnik APK Verxnevolzh`ya O V Ivanov D Kosterin Yu L E Mel`nikova 2019
13. Analiz izmenenij mikrobioma tolstogo otdela kishechnika svinomatok i porosyat // Agrarnaya nauka A V Filatov D N Ivanov A F Sapozhnikov 2024 11
14. Pleshakova V.I. Migraciya pticz kak faktor rasprostraneniya infekcionny`x agentov i antibiotikorezistentny`x shtammov mikroorganizmov // Vestnik Omskogo gosudarstvennogo agrarnogo universiteta E V Frik N A Leshheva 2025 2
15. Bioplenkoobrazuyushhaya mikroflora v strukture mikroorganizmov, vy`delenny`x ot sel`skoxozyajstvenny`x i domashnix zhivotny`x // Ucheny`e zapiski Kazanskoj gosudarstvennoj akademii veterinarnoj mediciny` im I V Antonevskij N.E`. Baumana. 2025. T. 261. №
16. S V I Pleshakova N.E`. Baumana. 2025. T. 261. №
17. S N A Leshheva N.E`. Baumana. 2025. T. 261. №
18. Shttps://doi.org/10.31588/2413-4201-1883-1-261-16
19. Issledovanie bioplenok mikroorganizmov pri disbakteriozax kishechnika u zhivotny`x // Problemy` veterinarnoj sanitarii, gigieny` i e`kologii E M Lenchenko D A Blyumenkrancz 2020 1
20. 10.36871/vet.san.hyg.ecol.202001009
21. Il`ina T.S., Romanova Yu.M. Bakterial`ny`e bioplenki: rol` v xronicheskix infekcionny`x processax i poisk sredstv bor`by` s nimi // Molekulyarnaya genetika, mikrobiologiya i virusologiya I V Antonevskij N.E`. Baumana. 2025. T. 264. №
22. S. V I Pleshakova N.E`. Baumana. 2025. T. 264. №
23. S. N A Leshheva N.E`. Baumana. 2025. T. 264. №
24. S.https://doi.org/10.31588/2413-4201-1883-4-264-6.10 2021. T. 39. №
25. S Modifikaciya metoda kolichestvennoj ocenki bioplenkoobrazuyushhej sposobnosti mikroorganizmov s ispol`zovaniem safranina // Ucheny`e zapiski Kazanskoj akademii veterinarnoj mediciny` im
26. Minenkova Ol'ga Vasil'evna, aspirant, ov.minenkova2412@omgau.org; Lorengel' Tat'yana Iosifovna, kand. vet. nauk, doc., ti.lorengel@omgau.org; Pleshakova Valentina Ivanovna, d-r vet. nauk, prof., vi.pleshakova@omgau.org Lescheva Nadezhda Alekseevna na.lescheva@omgau.org; * ; Antonevskiy Igor V., Cand. of Vet. Sci., Assistant, iv.antonevskiy@omgau.org; Minenkova Olga V., Postgraduate Student, ov.minenkova2412@omgau.org; Lorengel Tatyana I., Cand. of Vet. Sci., Ass. Prof., ti.lorengel@omgau.org; Pleshakova Valentina I., Doc. of Vet. Sci kand. vet. nauk, doc., na.lescheva@omgau.org * ; Antonevskiy Igor' Viktorovich, kand. vet. nauk, assistent, iv.antonevskiy@omgau.org Prof., vi.pleshakova@omgau.org. Stat'ya postupila v redakciyu 17.02.2026. The article was submitted 17.02.2026
