For the growth and development of meristemic plants, a balanced nutrient medium containing macro- and microelements, vitamins, carbohydrates and growth regulators that are in demand for plant morphogenesis is necessary. To improve morphometric parameters, we modified the nutrient medium by introducing into it a growth regulator, sucrose and agar in different concentrations and ratios (2 : 16 : 7; 3 : 20 : 6; 4 : 30 : 5 respectively). Morphometric parameters (height, number and length of internodes, number and length of roots) were evaluated on the 7th, 14th and 21st days of cultivation in varieties: Alyona, Nevsky, Kumir, Triumph, Bellarosa, Zhukovsky early. The research results showed that the most favorable concentration of the nutrient medium for plant growth and development was a ratio of 2 : 16 : 7 (MS-1), and for root formation - 4 : 30 : 5 (MS-3). The most internodes suitable for cuttings were formed in the early Zhukovsky variety (6.80 pcs.) at a late period of cultivation, and the roots - in the Triumph variety (10.25 pcs.). To check the relationship of plant height with the number and length of internodes and the number and length of roots, a correlation analysis was carried out, which showed a high correlation of height with the number of internodes and low correlation with other morphometric indicators.
potato, clonal microreproduction, nutrient medium, phytohormone, in vitro
1. Petrov A.F., Shul'ga M.S., Galeev R.R., Gavrilec N.V., Kolbina O.N. Sovershenstvovanie tehnologii proizvodstva kartofelya v usloviyah lesostepnoy zony Zapadnoy Sibiri putem optimizacii primeneniya organomineral'nyh stimulyatorov rosta // Innovacii i prodovol'stvennaya bezopasnost', 2022. № 2(36). S. 58-65. EDN: https://elibrary.ru/LJVIFH
2. Lebedeva N.V. Uskorennoe razmnozhenie rannih sortov kartofelya v usloviyah in vitro i ego ispol'zovanie v semenovodstve Severo-Zapada RF: dis. …kand. nauk / N.V. Lebedeva. Verhnie Luki, 2015. 188 s.
3. Lapshinov N.A., Kulikova V.I., Anoshkina L.S. Original'noe semenovodstvo kartofelya v usloviyah Kemerovskoy oblasti / V kn.: Kartofelevodstvo: sbornik nauchnyh statey. S.A. Turko (red.). Minsk: RUP "Nauchno-prakticheskiy centr Nacional'noy akademii nauk Belarusi po vyraschivaniyu kartofelya i ovoschey", 2013. S. 91-90.
4. Cheremisin A.I., Yakimova I.A., Elina A.M., Kling A.P. Otzyvchivost' sortov kartofelya na obrabotku mikroudobreniyami pri vyraschivanii rasteniy v teplice // Vestnik Omskogo gosudarstvennogo agrarnogo universiteta. 2021. № 4(44). S. 74-80. EDN: https://elibrary.ru/CHDDQA
5. Myakisheva E.P., Durnikin D.A., Tavartkiladze O.K. Vliyanie vitaminnogo morfogeneza na regenerirovannye rasteniya kartofelya in vitro dlya intensifikacii proizvodstva elitnogo posadochnogo materiala // Biologicheskiy vestnik Melitopol'skogo gosudarstvennogo pedagogicheskogo universiteta imeni Bogdana Hmel'nickogo. 2016. № 6(2). S. 166-173. EDN: https://elibrary.ru/XRJAUZ
6. Ryabceva T.V., Kulikova V.I., Hodaeva V.P. Ocenka pitatel'nyh sred dlya razmnozheniya sortov kartofelya in vitro // Mezhdunarodnyy issledovatel'skiy zhurnal. 2017. № 12-3(66). S. 134-137. EDN: https://elibrary.ru/YNMOCK
7. Dragan V., Ivana D., Branka V. Potato in vitro culture techniques and biotechnology. Global Science Books. 2017. P. 16-45.
8. Demenko V.I., Shestibratov K.A., Lebedev V.G. Ukorenenie - klyuchevoy etap vyraschivaniya sortov rasteniy in vitro // Izvestiya TSHA. 2010. № 1. S. 73- 85. EDN: https://elibrary.ru/LAJJIR
9. Yancheskaya T. Perspektivnaya tehnologiya optimizacii pervichnogo semenovodstva kartofelya // Nauka i innovacii. 2006. № 8(42). S. 37-40.
10. Jacobsen E., Hutten R. Stacking of resistance genes in potato by cisgenesis instead of introgression breeding. Potato developments in a changing Europ: Proc.Int. Symp. On potato (Germany, Hannover, September 8-10, 2006). Wagen, Acad. Publ., Netherlands, 2006. R. 41-68.
11. Cheremisin A.I., Kumpan V.N. Primenenie biopreparatov kompleksnogo deystviya i bioudobreniy v original'nom semenovodstve kartofelya // Vestnik Omskogo gosudarstvennogo agrarnogo universiteta. 2017. № 1(25). S. 28-34. EDN: https://elibrary.ru/YLGBIT
12. Petrasek J., Friml J. Auxin transport routes in plant development. Development. 2009;136:2675-2688.
13. Ewing E.E., Park (Eds.). W.D. C.A.B. Induction of tuberization in potato. In: The molecular and cellular biology of the potato.International, Redwood Press Ltd., Melksham. 2010; 25-41.
14. Metodika issledovaniy po kul'ture kartofelya. M.: VNIIKH, 1995. 105 s.
15. Metodika raboty s kul'turoy tkani // RASHN. 2002. № 6. 17 s.
16. Dospehov B.A. Metodika polevogo opyta. M.: Agropromizdat, 2011. 351s. EDN: https://elibrary.ru/QLCQEP
