The study focuses on a comparative analysis of the micromorphology of lymph nodes in the pelvic and abdominal cavities of clinically healthy California breed rabbits (n = 15). Using histological (hematoxylin-eosin staining), morphometric, and statistical methods, the structural features of the capsule, trabeculae, and hilum of lymph nodes in different locations (jejunal, mesenteric, iliac) were investigated. Histological analysis revealed a distinct layered structure of the capsule, composed of dense collagenous tissue and smooth muscle fibers, providing resistance to deformation. It was established that capsule thickness varies depending on anatomical location: maximum values were found in the mesenteric lymph node (9.78 ± 0.43 μm), while minimum values were observed in the iliac node (5.85 ± 0.59 μm), correlating with the intensity of mechanical stress during peristalsis. At the hilum, the capsule thickens by 24.9-55.4%, attributed not only to the presence of blood vessels, nerves, and the efferent lymphatic vessel but also to increased cellular density (fibroblasts, macrophages). The thickness of the connective tissue trabeculae differed between nodes (20.99 ± 0.77 - 23.18 ± 1.39 μm), reflecting stromal adaptation to dynamic loads and localized immune responses. The results demonstrate a correlation between the structural parameters of lymph nodes and their topography and functional activity, including lymph filtration and lymphoid follicle organization. The obtained data may be useful for developing diagnostic criteria for lymphatic system pathologies (lymphadenitis, neoplasms) in rabbits and for comparative analysis with other livestock species. The study highlights the importance of accounting for anatomical variability when interpreting biopsies in veterinary practice.
lymph nodes, rabbits, histology, morphometry, connective tissue, capsule, lymph node gate, comparative anatomy
1. Michelini L., Sternini A. Age-related changes in lymphatic function and adaptation // Journal of Vascular Research. 2007. Vol. 44, № 2. P. 115-125.
2. Rockson S.G., Miller L.T. Lymphatic insufficiency and the pathophysiology of lymphatic circulation // American Journal of Medicine. 1996. Vol. 102, № 3. P. 7-12.
3. Veremeeva S.A., Telenkov V.N. Limfaticheskie sosudy i uzly zheludka pushnyh zverey // Vestn. Michurinskogo gos. agrar. un-ta. 2019. № 1. S. 95-97. EDN: https://elibrary.ru/ZAIBHV
4. Morfo-adaptacionnye osobennosti limfaticheskih kapillyarov obodochnoy kishki krolikov raznyh vozrastnyh grupp / V.N. Nikiforov, V.N. Telenkov, G.A. Honin [i dr.] // Vestn. Omskogo gos. agrar. un-ta. 2024. № 4(56). S. 181-186. EDN: https://elibrary.ru/HKPRPR
5. Anatomo-topograficheskie osobennosti limfaticheskih sosudov i uzlov tazovoy i bryushnoy polosti u krolika / V.N. Nikiforov, V.N. Telenkov, G.A. Honin, V. A. Dem'yancev // Morfologiya v XXI veke: teoriya, metodologiya, praktika: sb. tr. Mezhdunar. nauch.-prakt. konf., Moskva, 24-26 apr. 2024 g. M.: FGBOU VO MGAVMiB-MVA im. K. I. Skryabina, 2024. S. 206-209. EDN: https://elibrary.ru/TYOVQL
6. Dem'yancev V.A., Telenkov V.N., Honin G.A. Mikromorfologiya vnutrennih organov razmnozheniya i ih vyvodnyh protokov u zayceobraznyh // Vestn. Omskogo gos. agrar. un-ta. 2024. № 1(53). S. 91-98. EDN: https://elibrary.ru/WQETGT
7. Skladneva E.Yu. Morfometricheskie harakteristiki limfaticheskih kapillyarov v usloviyah postnatal'nogo razvitiya // Morfologiya. 2012. T. 141, № 5. S. 78-84.
8. Ustinova T.I., Telenkov V.N., Nikiforov V.N. Morfologicheskie osobennosti limfaticheskih sosudov i uzlov golovy i shei u zhivotnyh // Aktual'nye voprosy sovremennoy morfologii, fiziologii i patologii: sb. tr. Nac. (Vseros.) konf., Tyumen', 14-15 noyab. 2024 g. Tyumen': GAU Severnogo Zaural'ya, 2024. S. 153-159. EDN: https://elibrary.ru/CCWLCG
9. Honin G. A. Morfologiya sosudov i nervov organov mochevydeleniya u pushnyh zverey kletochnogo soderzhaniya // Katalog nauch. i innovac. razrabotok FGBOU VO Omskiy GAU. Ser. "Veterinariya". Omsk: Omskiy GAU im. P.A. Stolypina, 2021. S. 174-175. EDN: https://elibrary.ru/BHMHFP
10. Mortimer P., Rockson S. New developments in the diagnosis and treatment of lymphatic diseases // New England Journal of Medicine. 2001. Vol. 345, № 13. P. 1023-1035.
