The aim of this work was to evaluate the effect of the AntiKlos feed additive (containing bacteria of the genus Bacillus and an organic acid) on hematological parameters, metabolic status, rumen digestion, and milk productivity of cows fed diets with a high level of contamination by opportunistic microflora. The study was conducted on a commercial dairy farm using two groups of lactating cows formed according to production principles, considering age and physiological state. The additive was included in the basal diet at a dose of 50 g/head/day for 30 days. For an in-depth study of hematological and biochemical parameters as well as rumen metabolism, 5 animals from each group were selected. Milk productivity was assessed in 15 cows from each group that remained in the experiment throughout the entire period, as well as for the groups as a whole (110 head each). Analysis of hematological parameters revealed restoration of the leukocyte formula, a decrease in antigenic load, and activation of erythropoiesis, indicating a positive effect of the additive on bone marrow hematopoiesis and immune status. It was also found that feeding the feed additive contributed to the stabilization of acid-base balance, correction of the functional state of the liver, and optimization of rumen metabolism, which was manifested in a decrease in ammonia concentration and regulation of the volatile fatty acids ratio. In the experimental group, a tendency to maintain milk productivity against the background of a physiological decline in lactation (difference with the control 0.56–0.62 L/day) was noted, as well as a significant increase in milk quality – mass fraction of fat, dry matter, and solids-not-fat (SNF) (p < 0.01).
lactating cows, antiklos feed additive, feed contamination, opportunistic microflora
1. Petrov K.S., Sidorova E.N. Sohrannost' zagotovlennyh kormov: osnovnye faktory riska // Izv. Timiryazevskoy sel'skohozyaystvennoy akademii. 2019. № 4. S. 45–56.
2. Garcia S., Vargas R.O., Fernandez L.M. Microbial Safety of Animal Feed: A Review of Current Status and Future Perspectives // Frontiers in Veterinary Science. 2021. Vol. 8. Art. 689195. DOI:https://doi.org/10.3389/fvets.2021.689195.
3. Feddern V., Mazzuco H., Fonseca F.N. A review on biogenic amines in food and feed: toxicological aspects, impact on health and control measures // Animal Production Science. 2019. Vol. 59, No. 4. P. 608–618. DOI:https://doi.org/10.1071/AN17676.
4. Impact of food processing and detoxification treatments on mycotoxin contamination / P. Karlovsky, M. Suman, F. Berthiller [et al.] // Mycotoxin Research. 2016. Vol. 32, No. 4. P. 179– 205. DOI:https://doi.org/10.1007/s12550-016-0257-7.
5. Effects of feeding mycotoxin-contaminated diets and the use of a yeast cell wall extracts mycotoxin adsorbent on ruminal and fecal microbiota of finishing beef steers / L.F. Batista, B.C. Agustinho, L.M. Deef [et al.] // Frontiers in Microbiology. 2025. Vol. 16. P. 1675653. DOI:https://doi.org/10.3389/fmicb.2025.1675653.
6. Krizek M. Possible ways of affecting biogenic amine content in silages // Veterinarni medicina. 1995. Vol. 40, No. 4. P. 111–115.
7. Besedin A.V., Golubkov A.I. Ekonomicheskie aspekty kormovoy bezopasnosti v zhivotnovodstve: monografiya. M.: Rosinformagroteh, 2020. 184 s.
8. Probiotik dlya snizheniya toksinov i povysheniya nadoya u korov / E.A. Yyldyrym, L.A. Il'ina, G.Yu. Laptev [i dr.] // Tr. Kubanskogo gosudarstvennogo agrarnogo universiteta. 2023.№ 107. S. 245–249. DOI:https://doi.org/10.21515/1999-1703-107-245-249.
9. AntiKlos: doyatsya bol'she, plodyatsya luchshe / G.Yu. Laptev, E.A. Yyldyrym, N.I. Novikova [i dr.] // Sel'skohozyaystvennye vesti. 2024. № 3. S. 22–27.
10. Vliyanie biologicheskoy kormovoy dobavki na mikrobiotu, pokazateli molochnoy produktivnosti i vosproizvodstva korov golshtinskoy porody / T.S. Smetannikova, V.A. Filippova, E.A. Yyldyrym [i dr.] // Agrarnyy vestnik Urala. 2024. T. 24, № 12. S. 1694–1704. DOI: https://doi.org/10.32417/1997-4868-2024-24-12-1694-1704.
