This article summarizes modern fundamental and experimental knowledge about the effectiveness, possible mechanisms and expediency of using an extract of plant origin as feed components. The object of the study was the cysts and Artemia nauplii (Artemia salina) of the Altai population. The aim of the study was to study the effect of grape seed proanthocyanide extract (GSPE) on the incubation activity and bioencapsulation properties of Artemia nauplii. The objective of the experiment was to investigate GSPE at concentrations of 7 and 14 mg/ml as an activator and antioxidant. The incubation of cysts was carried out by the standard method in Weiss devices without decapsulation. As a result of the experiments, an increase in the hatching of nauplius was found with a simultaneous increase in the antioxidant status. The hatching percentage was determined 24 hours and 36 hours after the start of incubation by counting free-floating nauplii in the field of view of a microscope. The yield of live Artemia nauplii ranged from 65 to 85%. The obtained samples of live artemia nauplius were examined for the total concentration of water-soluble antioxidants (TCWA) both in native form and after drying at 60 °C. The level of antioxidants in native samples was 0.575-0.661 mg/g, after drying - 3.08-3.64 mg/g. The results of the study expand knowledge about the impact of methods for obtaining live feed for aquaculture by adding ninety-five percent extract of grape seed proanthocyanides (Vitis vinifera L.) to the incubation solution. This makes it possible to increase the incubation efficiency and antioxidant activity of free-floating Artemia nauplii, which can increase the nutritional value of starter feeds for fish larvae.
grape seed proanthocyanide extract, artemia nauplii, live feed, antioxidants, sturgeon fish
1. The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. Rome: FAO, 2022.
2. Strategiya razvitiya rybhozyaystvennogo kompleksa Rossiyskoy Federacii na period do 2030 goda [Elektronnyy resurs] // Federal'noe agentstvo po rybolovstvu: [sayt]. URL: https://fish.gov.ru/otraslevaya-deyatelnost/ekonomika-otrasli/(data obrascheniya 15.07.2024).
3. Savkina L., Broun E. Otechestvennye korma dlya akvakul'tury. [Elektronnyy resurs] // Cenovik: [sayt] [2024]. URL: https://www.tsenovik.ru/articles/obzory-i-prognozy /otechestvennye-korma-dlya-akvakultury/?ysclid= lx8lxa08ci733611408 (data obrascheniya 19.06.2024).
4. Voloshin G.V., Akimov E.B., Artemov R.V., Gershunskaya V.V. Sostoyanie i perspektivy razvitiya rynka kombikormov dlya industrial'noy akvakul'tury v Rossiyskoy Federacii. Ekonomika, mezhdunarodnoe sotrudnichestvo i normativnye pravovye osnovy rybohozyaystvennoy deyatel'nosti // Trudy VNIRO. 2022. T. 190. S. 163-169. EDN: https://elibrary.ru/ZDVXMI
5. Portnaya T.V., Drugakova A.D. Zhivye korma v startovom kormlenii molodi osetrovyh // Aktual'nye problemy intensivnogo razvitiya zhivotnovodstva. 2018. № 21-1. S. 180-186. EDN: https://elibrary.ru/XSFQKL
6. Chebanov M.S., Galich E.V. Rukovodstvo po iskusstvennomu vosproizvodstvu osetrovyh ryb // Tehnicheskie doklady FAO po rybnomu hozyaystvu i akvakul'ture. № 558. Ankara: FAO. 2011. 297 s.
7. Azra M.N., Noor M.I.M., Burlakovs J. [et al.] Trends and New Developments in Artemia Research // Animals (Basel). 2022. № 12(18): 2321.
8. Sorgeloos P., Dhert P., Candreva P. Use of the brine shrimp, Artemia spp., in marine fish larviculture. Aquaculture. 2001. Vol. 200. P.147-159. EDN: https://elibrary.ru/ARAKKH
9. Wang Y., Zhang D., Zhang M. et al. Effects of ingested polystyrene microplastics on brine shrimp, Artemia parthenogenetica // Environ. Pollut. 2019. Vol. 244. P. 715-722.
10. Riisgård H.U., Jeune N., Pleissner D. [et al.] Adaptation of the brine shrimp Artemia salina (branchiopoda: Anostraca) to filter-feeding: Effects of body size and temperature on filtration and respiration rate //j. Crustac. Biol. 2015. Vol. 35. P. 650-658.
11. Henry J., Bai Y., Williams D. et al. Impact of test chamber design on spontaneous behavioral responses of model crustacean zooplankton Artemia franciscana // Lab. Anim. 2022. Vol. 51 P. 81-88. EDN: https://elibrary.ru/IVHANO
12. Aragão C., Conceição L.E., Dinis M.T., Fyhn H.-J. Amino acid pools of rotifers and Artemia under different conditions: Nutritional implications for fish larvae // Aquaculture. 2004. Vol. 234. P. 429-445. EDN: https://elibrary.ru/XSXMRO
13. Kryahova, N.V., Kovacheva N.P., Glazunov A.A. Vliyanie peroksida vodoroda na vyklev naupliev artemii Artemia sp. // Rybnoe hozyaystvo. 2023. № 3. S. 81-87. EDN: https://elibrary.ru/RCDSGC
14. Dey C.J., Tan Q.J., O'Connor C.M. [et al.] Dominance network structure across reproductive contexts in the cooperatively breeding cichlid fish Neolamprologus pulcher // Curr. Zool. 2015. Vol. 61. P. 45-54.
15. Hire J. Effect of probiotic, Bacillus spp. enriched artemia on growth of gold fish, Carassius auratus (Linnaeus, 1758) larvae. //j. Exp. Zool. 2020. Vol. 23. P. 741-745.
16. Hafezieh, M., Kamarudin, M.S., Saad, C.R.B. et al. Effect of enriched Artemia urmiana on growth, survival and composition of larval Persian sturgeon // Turkish Journal of Fisheries and Aquatic Sciences. 2009. № 9. P. 201-207.
17. Azimirad M., Meshkini S., Ahmadifard N., Hoseinifar S.H. The effects of feeding with synbiotic (Pediococcus acidilactici and Fructooligosa charide) enriched adult Artemia on skin mucus immune responses, stress resistance, intestinal microbiota and performance of angelfish (Pterophyllum scalare) // Fish Shellfish Immunol. 2016. Vol. 54. P. 516-522.
18. Rezul'taty primeneniya bioinkapsulirovannyh naupliy artemii v kachestve startovogo korma dlya lichinki i molodi sterlyadi / A.S. Elizarova, G.A. Shishanov, I.E. Lippo [i dr.] // Veterinariya i kormlenie. 2023. №5. S. 31-34. EDN: https://elibrary.ru/LYURRT
19. Prabakusuma A.S., Wardono B., Fahlevi M. [et al.] A bibliometric approach to understanding the recent development of self-sufficient fish feed production utilizing agri-food wastes and by-products towards sustainable aquaculture // Heliyon. 2023. № 9. e17573. EDN: https://elibrary.ru/ADWQKX
20. European Platform for the Responsible Use of Medicines in Animals (EPRUMA) 2018 [Elektronnyy resurs] // EPRUMA Best-Practice Framework for the Use of Antibiotics in Food-Producing Animals Reaching for the Next Level. 2018. URL: http://www.epruma.eu/ (data obrascheniya 11.06.2024).
21. Peng K., Chen B., Wang Y. [et al.] Condensed tannins protect against aflatoxin B1-induced toxicity in Lateolabrax maculatus by restoring intestinal integrity and regulating bacterial microbiota // Aquaculture. 2022. Vol. 555 738255. EDN: https://elibrary.ru/DIERWB
22. Costa J.R., Xavier M., Amado I.R. [et al.] Polymeric nanoparticles as oral delivery systems for a grape pomace extract towards the improvement of biological activities // Mater. Sci. Eng. C. 2021. Vol. 119:111551. EDN: https://elibrary.ru/NDGUSA
23. Farha A.K., Yang Q.-Q., Kim G. et al. Tannins as an alternative to antibiotics // Foo Biosci. 2020. Vol. 38: 100751.
24. Lavens P., Sorgeloos P. Manual on the production and use of live food for aquaculture FAO // Tech. Pap. 1996, 361. P. 295.
25. Fan X., Ma Y., inventor; Hospira. Method to hatch free swimming nauplii from artemia cysts. China patent. 101081015. 2007 Dec 05 URL: https://patents.google.com/patent/WO2008022569A1/en.
26. Gabetta B., Fuzzati N., Griffini A. [et al.] Characterization of proanthocyanidins from grape seeds // Fitoterapia. 2000. Vol. 71. P. 162-175.
27. Aysun C., Leylagul K., Ismail K. [et al.] Effect of grape seed extract on radiation-induced oxidative stress in rat liver // Turkey J. Gastroenterol. 2008. Vol. 19. P. 92-98.
28. Van S.G.Introduction, biology and ecology of Artemia // In: Manual on the production and use of live food for aquaculture. Belgium: Laboratory of Aquaculture and Artemia Reference Center, University of Ghent. 1996. P. 79-136.
29. Yashin A.Ya. Metodologiya opredeleniya antioksidantnoy aktivnosti pischevyh produktov i biologicheskih zhidkostey // Analitika. 2021. T 11. № 5. S. 370-371. EDN: https://elibrary.ru/HXTAIN
30. Navarro J.C., Amat F., Sargent J. R. Lipid composition of cysts of the brine shrimp Artemia sp. from Spanish populations //j. Exp. Mar. Biol. Ecol. 1992. Vol. 155. P. 123-131.
31. Yasuda A., Natsume M., Sasaki K. [et al.] Cacao procyanidins reduce plasma cholesterol and increase fecal steroid excretion in rats fed a high-cholesterol diet // Biofactors. 2008. Vol. 33. P. 211-223.
32. Tamura T., Inoue N., Ozawa M. [et al.] Peanut-skin polyphenols, procyanidin A1 and epicatechin-(4β→6)-epicatechin-(2β→O→7, 4β→8)-catechin, exert cholesterol micelle-degrading activity in vitro // Biosci Biotechnol Biochem. 2013. Vol. 77:1306-9.
33. Moreno D.A., Poulev A., Brasaemle D.L. et al. Inhibitory effects of grape seed extract on lipases // Nutrition. 2003. Vol. 19. P. 876-879.
34. Kiseleva T.F. Tehnologiya sushki: ucheb.-metod. kompleks // Kemerovskiy tehnologicheskiy institut pischevoy promyshlennosti. Kemerovo, 2007. 117 s.
