Domestic and foreign studies confirm the widespread use of mycotoxins in plant products, in many cases, the maximum permissible levels are exceeded. One of the regulated mycotoxins is ochratoxin A, which has pronounced toxic properties. In this regard, the article discusses the targets and mechanism of action of ochratoxin A and its potential hazard when accumulated in feed and food products. The action of ochratoxin A is realized due to hyperproduction of ROS and peroxidation of proteins and lipids with the development of mitochondrial dysfunction, disruption of protein synthesis and DNA damage, which can lead to the induction of apoptosis and necrosis. With ochratoxicosis, the function of renal epithelial cells is impaired, which causes the development of renal dysfunction (up to the development of renal failure). At the same time, the liver, cardiovascular and reproductive systems, respiratory organs, immune and central nervous systems are affected by the action of ochratoxin A. Given the toxicity of ochratoxin A, its content is standardized in animal feed, as well as in raw materials of plant origin and products made from it. Standardization of ochratoxin A is carried out in Russia (including within the Customs Union), countries of Europe and North America, as well as in most other countries of the world. Reducing the damage from ochratoxin A requires compliance with regulations on drying and storing grain. There are technologies that can reduce the amount of mycotoxins in feed ingredients and finished feed. The development of effective methods for detoxification and prevention of poisoning is of fundamental importance for maintaining health and ensuring the safety of all links in the food chain.
mycotoxicoses, ochratoxin a, mechanism of action, standardization, mycotoxicological monitoring
1. Dirheimer G. Mechanism of action of ochratoxin A / G. Dirheimer, E.E. Creppy // IARC Sci. Publ. 1991. № 115. P. 171-186.
2. Nutritional impact of mycotoxins in food animal production and strategies for mitigation / R. Xu, E.G. Kiarie, A. Yiannikouris [et al.] // J. Animal Sci. Biotechnol. 2022. Vol. 13. № 69. EDN: https://elibrary.ru/DLNCPK
3. Stoev S.D. Food security, underestimated hazard of joint mycotoxin exposure and management of the risk of mycotoxin contamination // Food Control. 2024. Vol. 159. P. 110235. EDN: https://elibrary.ru/NZTJMA
4. Economics of mycotoxins: evaluating costs to society and cost-effectiveness of interventions // IARC Sci. Publ. 2012. № 158. P. 119-129.
5. Pat. 2491942 S1 Rossiyskaya Federaciya, MPK A61K 35/00. Sposob profilaktiki mikotoksikozov i zheludochno-kishechnyh zabolevaniy zhivotnyh: № 2012106990/15: zayavl. 27.02.2012: opubl. 10.09.2013 / M.Ya. Tremasov, L.E. Matrosova, A.V. Ivanov, A.M. Tremasova [i dr.]; zayavitel' Federal'noe gosudarstvennoe byudzhetnoe uchrezhdenie "Federal'nyy centr toksikologicheskoy, radiacionnoy i biologicheskoy bezopasnosti".
6. Santos Pereira C., Cunha S.C., Fernandes J.O. Prevalent Mycotoxins in Animal Feed: Occurrence and Analytical Methods // Toxins (Basel). 2019. Vol. 11, № 5. P. 290.
7. Popova S.A., Skopcova T.I., Losyakova E.V Mikotoksiny v kormah: prichiny, posledstviya, profilaktika // Izv. Velikolukskoy gosudarstvennoy sel'skohozyaystvennoy akademii. 2017. № 1. S. 16-23. EDN: https://elibrary.ru/YMSAQJ
8. Abrunhosa L., Santos L., Venâncio A. Degradation of Ochratoxin A by proteases and by a crude enzyme of Aspergillus niger // Food Biotechnol. 2006. Vol. 20. P. 231-242.
9. Adsorption of Ochratoxin A and zearalenone by potential probiotic Saccharomyces cerevisiae strains and its relation with cell wall thickness / M.R. Armando, R.P. Pizzolitto, C.A. Dogi [et al.] // J. Appl. Microbiol. 2012. Vol. 113. P. 256-264.
10. Duarte S.C., Lino C.M., Pena A. Ochratoxin A in feed of food-producing animals: An undesirable mycotoxin with health and performance effects // Veterinary Microbiology. 2011. Vol. 154, № 1-2. P. 1-13.
11. Fisinin V., Suray P. Mikotoksiny i antioksidanty: neprimirimaya bor'ba. Ohratoksin A // Kombikorma. 2012. № 3. S. 55-60.
12. Dirheimer G. Mécanisme d'action de l'ochratoxine A, agent probable de la néphropathie endémique des Balkans / G. Dirheimer, R. Röschenthaler, E. Creppy // Bull. Acad. Natl. Med. 1983. Vol. 167, № 1. P. 67-74.
13. Balkan endemic nephropathy and associated urinary tract tumours: a review on aetiological causes and the potential role of mycotoxins / A. Pfohl-Leszkowicz, T. Petkova-Bocharova, I.N. Chernozemsky [et al.] // Food. Addit. Contam. 2002. Vol. 19, № 3. P. 282-302.
14. Krogh P. Role of ochratoxin in disease causation // Food and Chemical Toxicology. 1992. Vol. 30, № 3. P. 213-224.
15. Gekle M. Renal toxicodynamics of ochratoxin A: a pathophysiological approach / M. Gekle S. Silbernagl // Kidney Blood Press Res. 1996. Vol. 19, № 5. P. 225-35.
16. Microphysiological system modeling of ochratoxin A-associated nephrotoxicity / T. Imaoka, J. Yang, L. Wang [et al.] // Toxicology. 2020. Vol. 444. P. 152582. EDN: https://elibrary.ru/PYMSGE
17. Oxidative stress as a mechanism of combined OTA and CTN toxicity in rat plasma, liver and kidney / D. Rašić, V. Micek, M.Š. Klarić [et al.] // Hum. Exp. Toxicol. 2019. Vol. 38, № 4. P. 434-445.
18. Osobennosti proyavleniya hronicheskogo kormovogo mikotoksikoza u laboratornyh krys v usloviyah eksperimenta / M.P. Semenenko, E.V. Tyapkina, E.V. Kuz'minova [i dr.] // Sel'skohozyaystvennaya biologiya. 2019. T. 54, № 4. S. 777-786. EDN: https://elibrary.ru/QBYIVZ
19. Heussner A.H., Bingle L.E.Comparative Ochratoxin Toxicity: A Review of the Available Data // Toxins (Basel). 2015. Vol. 7, № 10. P. 4253-82.
20. The Neurotoxic Effect of Ochratoxin-A on the Hippocampal Neurogenic Niche of Adult Mouse Brain / E. Mateo, R.P.B. Tonino, A. Canto [et al.] // Toxins (Basel). 2022. Vol. 14, № 9. P. 624. EDN: https://elibrary.ru/DXJVPR
21. Al-Anati L., Petzinger E. Immunotoxic activity of ochratoxin A // J. Vet. Pharmacol. Ther. 2006. Vol. 29, № 2. P. 79-90.
22. Effects of Subcutaneous Ochratoxin-A Exposure on Immune System of Broiler Chicks / S.A. Khan, E.J. Venancio, M.A. Ono [et al.] // Toxins (Basel). 2019. Vol. 11, № 5. P. 264.
23. Ochratoxin A affects oocyte maturation and subsequent embryo developmental dynamics in the juvenile sheep model / M.E. Dell'Aquila, S. Asif, L. Temerario [et al.] // Mycotoxin Res. 2021. Vol. 37, № 1. P. 23-37. EDN: https://elibrary.ru/QRNXVS
24. Melatonin ameliorates ochratoxin A-induced oxidative stress and apoptosis in porcine oocytes / M. Lan, Y. Zhang, X. Wan [et al.] // Environ. Pollut. 2020. Vol. 256. P. 113374. EDN: https://elibrary.ru/QGLZCK
25. Aydin G. Histopathologic changes in liver and renal tissues induced by Ochratoxin A and melatonin in rats / G. Aydin, N. Ozçelik, E. Ciçek [et al.] // Hum. Exp. Toxicol. 2003. Vol. 22, № 7. P. 383-91.
26. Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits / Z. Zhang, J. Xu, X. Zhang [et al.] // Toxins (Basel). 2022. Vol. 14, № 9. P. 628. EDN: https://elibrary.ru/UGJWLP
27. Protective Effects of Astaxanthin on Ochratoxin A-Induced Liver Injury: Effects of Endoplasmic Reticulum Stress and Mitochondrial Fission-Fusion Balance / Y. Zou, S. Zhang, J. Yang [et al.] // Toxins (Basel). 2024. Vol. 16, № 2. P. 68. EDN: https://elibrary.ru/UONADN
28. Effects of ochratoxin A on membrane phospholipids of the intestine of broiler chickens, practical consequences / I. El Cafsi, S. Bjeoui, I. Rabeh [et al.] // Animal. 2020. Vol. 14, № 5. P. 933-941. EDN: https://elibrary.ru/YLWYQK
29. Effects of ochratoxin a on broiler leukocytes / M.A. Moura, C.H. Machado, L. Porfirio [et al.] // Braz. J. Poult. Sci. 2004. Vol. 6, № 3. P. 191-194.
30. Regional difference in the neurotoxicity of ochratoxin A on the developing cerebral cortex in mice / T. Miki, Y. Fukui, N. Uemura [et al.] // Brain Res. Dev. Brain Res. 1994. Vol. 82, № 1-2. P. 259-64.
31. Ochratoxin A induces locomotor impairment and oxidative imbalance in adult zebrafish / J. Valadas, A. Sachett, M. Marcon [et al.] // Environ. Sci. Pollut. Res.Int. 2023. Vol. 30, № 8. P. 21144-21155. EDN: https://elibrary.ru/HCXEZM
32. Ochratoxin A induces liver inflammation: involvement of intestinal microbiota / W. Wang, S. Zhai, Y. Xia [et al.] // Microbiome. 2019. Vol. 7, № 1. P. 151. EDN: https://elibrary.ru/BVMOWT
33. Effect of ochratoxin A on the intestinal mucosa and mucosa-associated lymphoid tissues in broiler chickens / C. Solcan, G. Pavel, V.C. Floristean [et al.] // Acta Vet. Hung. 2015. Vol. 63, № 1. P. 30-48.
34. Moskva E.M., Belysheva L.L. Opredelenie ohratoksina A v pischevyh produktah // Zdorov'e i okruzhayuschaya sreda. 2014. № 24-2. S. 210-212. EDN: https://elibrary.ru/ZBJZKT
35. Naumenko N.V., Botvinnikova V.V. Issledovanie riskov kontaminacii zernovyh kul'tur mikotoksinami toksigennyh pleseney // Vestnik Yuzhno-Ural'skogo gosudarstvennogo universiteta. Seriya: Pischevye i biotehnologii. 2020. T. 8, № 2. S. 74-81. EDN: https://elibrary.ru/GGXVDP
36. Analitika dannyh obschey toksichnosti kormov / Z. H. Sagdeeva, E.I. Semenov, A.R. Valiev [i dr.] // Sovremennye problemy eksperimental'noy i klinicheskoy toksikologii, farmakologii i ekologii: sb. tez. dokl. Mezhdunar. nauch.-prakt. konf., Kazan', 09-10 sent. 2021 g. Kazan': Federal'nyy centr toksikologicheskoy, radiacionnoy i biologicheskoy bezopasnosti, 2021. S. 129-133. EDN: https://elibrary.ru/BLMDNG
37. Gerunova L.K, Gerunov V.I., Korneychuk D.V. Profilaktika mikotoksikozov v zhivotnovodstve // Vestnik Omskogo gosudarstvennogo agrarnogo universiteta. 2018. № 3(31). S. 36-43. EDN: https://elibrary.ru/VAPMOW
38. Izosimova I.V., Aksenov I.V. Izuchenie soderzhaniya ohratoksina A v produktah pitaniya, izgotovlennyh na osnove zerna kukuruzy // Uspehi medicinskoy mikologii. 2015. T. 14. S. 296. EDN: https://elibrary.ru/WGWHHF
39. Aspergillus steynii and Aspergillus westerdijkiae as potential risk of OTA contamination in food products in warm climates / J. Gil-Serna, B. Patiño, L. Cortes [et al.] // Food Microbiology. 2015. Vol. 46. P. 168-175.
40. Diagnostika i veterinarnaya pomosch' pri otravleniyah zhivotnyh: (Obschie principy) / A.M. Tremasova, I.I. Idiyatov, E.I. Semenov [i dr.]. Kazan': Federal'nyy centr toksikologicheskoy, radiacionnoy i biologicheskoy bezopasnosti, 2022. 236 s. EDN: https://elibrary.ru/MVJOMR
